Coding
Part:BBa_K634000:Design
Designed by: Eric Walters Group: iGEM11_Wisconsin-Madison (2011-09-12)
exaD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 158
Illegal AgeI site found at 305 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The synthesis included a Shine-Dalgarno-complimentary RBS 5' of the gene.
Source
De novo synthesis, codon optimized for E. coli. Original amino acid sequence taken from Pseudomonas aeruginosa.