Coding

Part:BBa_K634000:Design

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-12)


exaD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 158
    Illegal AgeI site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The synthesis included a Shine-Dalgarno-complimentary RBS 5' of the gene.


Source

De novo synthesis, codon optimized for E. coli. Original amino acid sequence taken from Pseudomonas aeruginosa.

References